The single-dose staggered start oral toxicity of Earthbind™ 100 was evaluated in Sprague Dawley rats. Initially, one female rat received a single oral administration of the test article at a dose of 5000 mg/kg body weight. Since the initial animal showed no signs of mortality, two additional female rats received the test article, a dose of 5000 mg/kg body weight. Following dosing, the limit test rats were observed daily and weighed weekly. A gross necropsy examination was performed on all limit test animals at the time of scheduled euthanasia (day 14).
No mortality occurred during the limit test. Clinical abnormalities observed during the study included dark material around the eye(s) and ocular lesion(s). Slight body weight loss was noted in one female during the study day 7 to 14 body weight interval. Body weight gain/maintenance was noted for all other animals during the test period. All animals exceeded their initial body weight by study termination (day14). No significant gross internal findings were observed at necropsy on study day 14.
Under the conditions of this test, the acute oral LD50 of Earthbind™ 100 was estimated to be greater than 5000 mg/kg in the rat. The results of the study indicated that the test material would be assigned an EPA-OPPTS Toxicity Category IV.
Complete test procedure and data are available upon request.